CRISPR-Adventskalender

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© Daria Chrobok, DC SciArt

Crop: Wine (Vitis vinifera)
Property: Tolerance against grey mold

Grey mold ("Botrytis cinerea") is a widespread mold that infests over 2,000 host plants, including grapevine. In viticulture, problems with Botrytis cinerea can occur in damp weather or when grapes are damaged, for example as a result of hail or heavy rainfall. If the fungus attacks the unripe grapes, it causes a loss of yield and quality. Soft-skinned varieties such as Müller-Thurgau and Riesling are particularly susceptible. In conventional cultivation, infestation can occur despite preventive fungicide applications or despite the use of potassium hydrogen carbonate as a last resort. Scientists at Northwest A&F University in China have used the CRISPR/Cas technique to specifically generate a point mutation in a gene known for its role in biotic stress response. The results of the experiment showed an increased tolerance to grey mold in the edited plants. Such approaches can reduce the use of pesticides and contribute to more environmentally friendly agriculture.

Publication

TitleCRISPR/Cas9-mediated efficient targeted mutagenesis in grape in the first generation
AuthorsXianhang Wang et al.
CountryChina
JournalPlant Biotechnology Journal
Year2017
DOIdoi:10.1111/pbi.12832